![]() ![]() ![]() Cell density is approximately equal to that of Fig. The 10-fold viral dilution reduces the amount of infectious virus present, thereby reducing the number of infected mouse L cells and providing a field of view with a countable number of fluorescing cells. ![]() 1.įigure 3: Mouse L cells infected with a 1/10 viral dilution and viewed with epifluorescence at 200x magnification. This image shows the same field of view as Fig. A majority of the cells are infected and display a distinct cytoplasmic fluorescence. ![]() Cells infected with virus are first tagged with a rabbit anti-reovirus antibody and subsequently tagged with a secondary anti-rabbit antibody, which is fluorescently labeled. Maximal viral production within an infected cell occurs about 24 hours postentry.įigure 2: Infected mouse L cells viewed with epifluorescence at 200x magnification. After assembly of new viral particles, host cells will be lysed to release new infectious viral particles. Proteolysis of the outer-capsid proteins occurs via a two-step disassembly process and replication then occurs in the cytoplasm. Following virion attachment to cell surface receptors, reovirus particles enter the host cell through receptor-mediated endocytosis. Download the PowerPoint PowerPoint Contentsįigure 1: Infected mouse L cells viewed with bright-field microscopy at 200x magnification. ![]()
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